This study will examine bacteria and toxins in the mouth, lung and digestive system that may be the cause of various diseases or symptoms. H. pylori is a bacterium that produces various toxins that may contribute to lung problems. This study will examine specimens collected from the mouth, teeth, lung, digestive tract and blood to measure H. pylori and its toxins and their effects on cells.

People 18 years of age and older with or without gastrointestinal disease may be eligible for this study. These include people without a history of lung disease as well as patients with any of the following: lymphangioleiomyomatosis, asthma, sarcoidosis, other chronic or genetic lung disease (e.g., chronic obstructive pulmonary disease, cystic fibrosis or eosinophilic granuloma).

Participants may undergo the following tests:

• Blood and urine tests, chest x-ray.
• Measurement of arterial blood gases: A small needle is placed in an artery in the forearm to collect arterial blood.
• Lung function tests: Subjects breathe deeply and occasionally hold their breath. They may also receive a medication that expands the airways.
• Fiberoptic bronchoscopy with lavage and bronchial brushing: The subject's mouth and throat are numbed with lidocaine; a sedative may be given for comfort. A thin flexible tube called a bronchoscope is advanced through the nose or mouth into the lung airways to examine the airways. Saline (salt water) is then injected through the bronchoscope into the air passage and then removed by gentle suction. Next, a small brush is passed through the bronchoscope and an area of the airway is brushed to collect some cells for examination.
• Mouth rinsing or teeth brushing to collect cells.
• Endoscopy: A small needle and catheter (thin plastic tube) are placed into an arm vein to administer fluids and medications through the vein. A sedative may be given. The throat is numbed with lidocaine and a thin flexible tube called an endoscope is inserted through the mouth and down the esophagus into the stomach and upper part of the small intestine to examine those areas.

Vacuolating cytotoxin A (VacA toxin), an 88-kDa multifunctional protein, and other toxins are produced by Helicobacter pylori. We hypothesize that H. pylori, VacA toxin, and other toxins within the gastrointestinal tract and/or oropharynx are also found in the lung and may contribute to decline in lung function. Analyses of gastrointestinal, oropharyngeal, lung and blood specimens will improve the understanding of H. pylori, VacA toxin, and other toxins as well as their potential role in pathophysiology of disease. The objectives of this exploratory protocol are to procure gastrointestinal, oropharyngeal, lung and/or blood specimens from healthy research volunteers and subjects with lung disease (e.g., lymphangioleiomyomatosis, asthma, sarcoidosis, pulmonary fibrosis) and to analyze these specimens for H. pylori, VacA toxin, and other toxins. We hypothesize that the toxins may have a role in the pathogenesis of lung disease and in the subclinical decline in lung function seen with aging.

• INCLUSION CRITERIA:

Individuals who are 18 years of age or older with or without a history of gastrointestinal disease and with any of the following:

1. lymphangioleiomyomatosis, or
2. asthma, or
3. sarcoidosis, or
4. pulmonary fibrosis, or
5. other chronic or genetic lung diseases (e.g., chronic obstructive pulmonary disease, eosinophilic granuloma, cystic fibrosis, Wegener's granulomatosis, chronic bronchitis), or
6. research volunteers without a history of lung disease.

EXCLUSION CRITERIA:

Individuals with any of the following:

1. uncontrolled ischemic heart disease, or
2. uncorrectable bleeding diathesis, or
3. pregnancy or lactation, or
4. inability to give informed consent, or
5. risk factors for endocarditis (e.g., prosthetic cardiac valve, previous bacterial endocarditis, surgically constructed systemic pulmonary shunts or conduits, complex cyanotic congenital heart disease [e.g., single ventricle, transposition of great arteries, tetralogy of Fallot])

139 patient volunteers and 18 healthy research volunteers have participated in this protocol at the NIH. 133 female patients with lymphangioleiomyomatosis (age range at accrual from 22 to 72 years old: 121 white, 7 black, 4 asian, and 1 unknown). 2 female patients with other chronic/genetic lung diseases (age range at accrual from 47 to 71: 2 white). 4 male patients with pulmonary fibrosis (age range at accrual from 67 to 74: 4 white). 18 research volunteers without lung disease (age range at accrual from 19 to 67: 6 white males, 5 white females, 5 black females, 1 hispanic multi-race male, and 1 hispanic unknown male.